PreTect HPV-Proofer is an assay for the qualitative detection of the presence of Human Papillomavirus, HPV, E6/E7 oncogene mRNA from carcinogenic HPV types 16, 18, 31, 33 and 45. ISC (intrinsic sample control, used to prove viability of the eluate) is included in order to assess specimen quality and reveal possible factors that may inhibit the amplification. The kit is based on real-time NASBA technology. PreTect HPV-Proofer is ideal having high specificity in triage of ASC-US and LSIL cytology diagnosis. PreTect HPV-Proofer is a valuable tool for identifying women having high risk of severe dysplasia and at the same time reducing unnecessary referral costs and psychological stress.
Platform/instrument: Fluorescence reader. Please contact PreTect for information on supported readers.
Software: PreTect Analysis Software (PAS)
PreTect HPV-Proofer is also available in plate format to facilitate higher volumes of samples. Find more information by viewing the PreTect HPV-Proofer HV page.
Please note that Isolation Reagents for Nucleic acids are not included in the kit. RNA (or total nucleic acids) from the collected samples have to be isolated prior to analysis. PreTect X and PreTect X Lysis Buffer are validated against this assay.
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